ROM-CRD Hemiptera

November 12, 2025

Sample information
Picture
Photos by: Chloe D.
Location
Collection date 08/27/2025
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

Sucking mouth part, Brown, antennas
Putative identification Arthropoda Insecta Hemiptera

Methods
Extraction kit
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

I used positive and negative DNA to compare my sample too and it came back matching the positive. On blast my sample has 100% match to a Wolbachia sample.
Wolbachia 16S sequence
Arthropod COI sequence
ATCTTCAATAAGATGAATTATCCGAATTGAATTAGGACAACccgtaacgt ttATTGGAAATGATCAAATTTATAATGTTATTGTTACtgtacATGCATTT ATTATAATTTTTTTCATAGTTATACCAATCATAATTGGAGGATTTGGAAA TTGATTAATCCCTTTAATAATTGGAGCCCCTGATATAGCATTCCCCCGAA TAAATAATATAAGATTTTGATTATTACCCCCCTCATTGACTTTATTATTA ATAAGAAGAATAGTAGAAATAGGAGCAGGAACAGGATGAACTGTATACCC TCCTTTATCTAATTCTATATTTCACAGAGGAGCCCCAGTAGATATAGCAA TTTTTTCTTTACATTTAGCAGGTATTTCATCAATCTTAGGAGCTATTAAT TTCATTTCAACAATTATTAATATACGACCATCAGGTATAACTCCTGAAAA GACACCACTATTTGTATGATCAGTTGGTATTACTGCTCTATTACTATTAT TATCATTACCAGTATTAGCTGGAGCCATCACTATACTATTAACCGACCGA AATTTCAATACTTCATTTTTTGATCCAACAGGAGGAGGAGACCCTATTCT ATATCAACATCTATTTTGATTTTTTGGTC
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Hemiptera was found to be postive for Wolbachia.
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