Sample information |
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| Picture |
|
|---|---|
| Location | |
| Collection date | |
| Captive / Cultivated? | Wild-caught |
| Group | Georgia Southern University |
| Observations |
|
| Putative identification | Arthropoda |
Methods |
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| Extraction kit | |
| DNA extraction location | Partial abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | Other |
| Gel images |
|
| Protocol notes | DNA extraction kit of in-house reagents was used. |
Results |
|
| Wolbachia presence | Unknown |
| Confidence level | Medium |
| Explanation of confidence level | Wasn’t really confident from the start. When the results came out as unknown though it honestly made me even more confused. I honestly cannot trust my results. |
| Wolbachia 16S sequence |
GTTCATCTTTAAGATGAATTATTCGTATTGAattcgttcAACCAGGGGTATTTATTGGAGATGATCAAATTTATAATAGTATTGTTACTGCACATGCATTTATTATAATTTTTTTTATAGTTATACCAATTATAATTGGAGGATTTGGAAATTGATTAGTGCCTTTAATAATTGGGGCCCCAGATATAGCATTCCCACGAATAAATAATATAAGATTTTGATTACTTCCACCATCTATTACTTTATTAATTAGAAGAAGATTAATTGAAATAGGAGCAGGGACAGGATGAACAGTTTATCCTCCCCTTTCTAACAATTTATTTCATAGAGGAGCATCAGTAGATATGGCAATTTTTTCATTACATTTAGCAGGAGTTTCATCAATTTTAGGGGCTATTAATTTTATCTCAACAATTATTAATATACGCCCATCAGGTATATCTCTTGAACAAACACCACTATTTGTATGATCAGTTGGAATTACTGCTTTATTATTATTATTATCTTTACCTGTATTAGCAGGAGCTATTACTATGCTTCTAACTGATCGAAATTTTAATACATCATTCTTTGACCCTACGGGAGGAGGGGATCCTATCTTGTATCAACATTTATTTTGATTTTTTGGT
BLAST at The Wolbachia Project BLAST at NCBI
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| Arthropod COI sequence |
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| Summary | |
Common Eastern Bumble Bee (Bombus impatiens)
American Bird
Spotted crane fly
Wolbachia data
Meadow Katydid