VA2 – Pill Bug

November 16, 2025

Sample information
Picture
Entry by: Varun A.
Location
Collection date 11/07/2025
Captive / Cultivated? Wild-caught
Group Walton High School
Observations

Pill Bug. Slow movements under the pinestraw. Hard exoskeleton. Gray-Black on top and white on bottom.
Putative identification Arthropoda Malacostraca Isopoda Armadillidae Armadillidium Armadillidium vulgare

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniPCR
Buffer 1X TAE
DNA stain GelGreen
Gel images
Protocol notes
  • Got DNA from the abdomen of the pillbug.
  • Finished extracting DNA, now need to conduct PCR.

Results
Wolbachia presence Unknown
Confidence level High
Explanation of confidence level

I did not see a band. Also the quality score was pretty low for this sample, so I don’t know how good my DNA sample was therefore, it is unknown for whether this was Wolbachia positive.
Wolbachia 16S sequence Download FASTA   
Arthropod COI sequence Download FASTA   
Summary
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