VA2 – Pill Bug

November 16, 2025

Sample information
Picture
Photos by: Varun A.
Location
Collection date 11/07/2025
Captive / Cultivated? Wild-caught
Group Walton High School
Observations

Pill Bug. Slow movements under the pinestraw. Hard exoskeleton. Gray-Black on top and white on bottom.
Putative identification Arthropoda Malacostraca Isopoda Armadillidae Armadillidium Armadillidium vulgare

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Abdomen
Single or Duplex PCR
Gel electrophoresis system
Buffer
DNA stain
Gel images
Protocol notes
  • Got DNA from the abdomen of the pillbug.
  • Finished extracting DNA, now need to conduct PCR.

Results
Wolbachia presence
Confidence level
Explanation of confidence level
Wolbachia 16S sequence
Arthropod COI sequence
Summary
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