Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 09/04/2025 |
| Captive / Cultivated? | Wild-caught |
| Group | Benedictine University |
| Observations | Collected by Fall 2025 students, found on concrete |
| Putative identification | Arthropoda Insecta Hymenoptera |
Methods |
|
| Extraction kit | DNeasy (Qiagen) blood and tissue kit |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | SYBR Safe |
| Gel images |
|
| Protocol notes | |
Results |
|
| Wolbachia presence | Yes |
| Confidence level | Medium |
| Explanation of confidence level | You can see the distinct bands of DNA, as well as a DNA ladder that is visible. The bands are also generally well separated, suggesting the gel concentration and running were adequate. |
| Wolbachia 16S sequence |
TAACAAGGTGTGGCACAGCGTTGAACTCACGCGCGTTTCAAGTCCCCTGGGAGTTTAA
BLAST at The Wolbachia Project BLAST at NCBI
|
| Arthropod COI sequence |
TCTTTGGTCTATGAGCTGGTATAATTGGAAGATCATTAAGATTAATTATTCGTTTAGAATTAGGAACACCTTTATCAATTATTAATAATGATCAAATCTATAATTCAATTATTACAGCTCACGCATTAATTATAATTTTTTTCATAGTTATACCATTTATAATTGGAGGATTTGGAAATTGATTAATTCCTTTAATATTAGGTGCCCCAGATATAGCATTTCCACGAATAAATAATATAAGATTTTGATTATTACCACCATCATTAATATTATTAATTATTAGAAATATTAACGATATAGGAGTAGGAACTGGATGAACTTTATATCCACCTTTATCATCTAATATAGGTCACAATTCATCATCAGTTGATTTTGCTATTTTCTCTCTTCACATTGCAGGAATTTCATCAATTATAGGAGCTATTAATTTCATTGTAACAATCTTAAATATACATATTAAAACTCATTCATTAAATTTTTTACCTATATTTACATGATCAGTTTTAATTACTGCAATTTTACTTTTATTATCTTTACCTGTATTAGCTGGAGCAATTACAATACTATTAACCGATCGAAATATTAATACATCTTTTTTTGATCCATTAGGAGGTGGAGACCCTATTTTATTCCAACATTTATTTTGATTTTTTGGTCANNNNNNNAANNTTTNAAA
BLAST at The Wolbachia Project BLAST at NCBI
|
| Summary | The Hymenoptera was found to be postive for Wolbachia. |
Common Eastern Bumble Bee (Bombus impatiens)
American Bird
Spotted crane fly
Wolbachia data
Meadow Katydid