Sample information |
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| Picture |
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|---|---|
| Location | |
| Collection date | 09/02/2025 |
| Captive / Cultivated? | Wild-caught |
| Group | Benedictine University |
| Observations | |
| Putative identification | Arthropoda Insecta Hymenoptera |
Methods |
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| Extraction kit | DNeasy (Qiagen) blood and tissue kit |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | SYBR Safe |
| Gel images |
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| Protocol notes | |
Results |
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| Wolbachia presence | No |
| Confidence level | Low |
| Explanation of confidence level | My controls worked, but as seen in the gel no second band for Wolbachia is present |
| Wolbachia 16S sequence | |
| Arthropod COI sequence | Download FASTA
Download AB1
TTTTATCAATAGAAAAATTTTTATGATGTATATTAATAATTGTTGAAATAAAATTAATAGCCCCTAAAATAGATGATATA CCGGCAATATGAAGAGAAAAAATTGTTAAGTCTACAGAAGGTCCATTATGAAAAATATTTGAAGCTAAAGGAGGGTAGAT AGTTCATCCAGTTCCAGTACCATCATTAATAAAGTTTCTTAAAAGTAATAGAGTGATGGAAGGAGGTAAAAGTCAAAATC TTATATTATTTATACGAGGATAAGCTATATCTGGTGACCCTAATATTAAAGGAATTAGAAAATTTCCAAATCCACCAATT ATAAATGGTATTACTATAAAAAAGATTATAATAAAGGCATGATTAGTTACTAAAGAATTATAAATTTGATCATTATTAAT TAATGAATTTGAAGAACCTAATTCTAATCGAATAATTATACTTATAGAAGATCCAATTATTCCAGCTCA
BLAST at The Wolbachia Project BLAST at NCBI
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| Summary | The Hymenoptera was found to be negative for Wolbachia. |
Common Eastern Bumble Bee (Bombus impatiens)
American Bird
Spotted crane fly
Wolbachia data
Meadow Katydid