Portland State University – Curculionidae

December 3, 2025

Sample information
Picture
Photos by: Maya N.
Location
Collection date 10/09/2025
Captive / Cultivated? Wild-caught
Group Portland State University
Observations
Putative identification Arthropoda Insecta Coleoptera Curculionidae

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Partial abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer 1X TAE
DNA stain GelRed
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

Gel B, wells 11-13. Well 11 is positive for insect DNA, well 12 is positive for Wolbachia positive control, and well 13 is negative for Wolbachia in the weevil.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Curculionidae was found to be negative for Wolbachia.
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