Portland State University – Cicadellidae

December 3, 2025

Sample information
Picture
Photos by: Jason F.
Location
Collection date 10/16/2025
Captive / Cultivated? Wild-caught
Group Portland State University
Observations
Putative identification Arthropoda Insecta Hemiptera Cicadellidae

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer 1X TAE
DNA stain GelRed
Gel images
Protocol notes

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

Gel A, wells 20-22. Well 20 is positive for insect DNA, well 21 is positive for Wolbachia positive control, and well 22 is positive for Wolbachia in the bug.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Cicadellidae was found to be postive for Wolbachia.
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