Portland State University – Nebria brevicollis

December 3, 2025

Sample information
Picture
Photos by: Callie V.
Location
Collection date 10/08/2025
Captive / Cultivated? Wild-caught
Group Portland State University
Observations
Putative identification Arthropoda Insecta Coleoptera Carabidae Nebria Nebria brevicollis

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Partial abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer 1X TAE
DNA stain GelRed
Gel images
Protocol notes

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

Gel B, wells 2-4. Well 2 is positive for insect DNA, well 3 is positive for Wolbachia positive control, and well 4 is positive for Wolbachia in the beetle.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Nebria brevicollis was found to be postive for Wolbachia.
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