Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 09/26/2025 |
| Captive / Cultivated? | Wild-caught |
| Group | NCSSM |
| Observations | Caught with UV light behind sheet. |
| Putative identification | Arthropoda Insecta Coleoptera Staphylinidae |
Methods |
|
| Extraction kit | DNeasy (Qiagen) blood and tissue kit |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | MiniPCR |
| Buffer | TBE |
| DNA stain | SYBR Green |
| Gel images |
|
| Protocol notes | |
Results |
|
| Wolbachia presence | No |
| Confidence level | Medium |
| Explanation of confidence level | Wolbachia strain could be present within arthropod that does not bind with the primers we used. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
TTAATATTCTTGGTGATAATTACCAATTATATAATGTAATAGTTACAAGTCATGCTTTGGTTATGGTATT TTTTATGATTATGCCAGCTCTAATGGGTGGCTTTGGTAATTGGTTTGTTCCTCTTATGATAGGTGCACCG GATATGGCTTTTCCGCGTATGAATAATTTGAGCTTTTGGCTACTGATATCATCCTTTGTCTTGCTCATTA TGTCTCTGTTTATTGGCGAAGGAGCAGGTACTGGTTGGACGTTATACCCACCACTATCACAAGTTAATTC CCATCCTAGTGCAGCTGTTGATCTGACAATATTTGCACTTCATGTTGCTGGGGTCTCCTCAATTGTTGGT GCTATTAATTTTATCGTTACTATATTTAATATGCGCACTCCTGGGATGACCTTGCATAAGATGCCTTTAT TTGTTTGGTCTATTTTACTTACCGCTTTTATGCTCATAGTTGCTTTGCCAGTATTAGCTGGAGCTATCAC TA
BLAST at The Wolbachia Project BLAST at NCBI
|
| Summary | The Staphylinidae was found to be negative for Wolbachia. |
American Bird
Spotted crane fly
Wolbachia data
Meadow Katydid
Blattella germanica