Common Eastern Bumble Bee (Bombus impatiens)

December 4, 2025

Sample information
Picture
Location
Collection date 09/02/2025
Captive / Cultivated? Wild-caught
Group Benedictine University
Observations

Collected by Fall 2025 students using swap net. Found in flowering bush plant low to the ground.
Putative identification Arthropoda Insecta Hymenoptera Apidae Bombus Bombus impatiens

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level Low
Explanation of confidence level

Barely visible/ very weak Wolbachia gel band was visible after the electrophoresis lab.
Wolbachia 16S sequence Download AB1
Arthropod COI sequence
Summary The Bombus impatiens was found to be negative for Wolbachia.
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