Wolbachia Project (Bombus Terrestris)

December 5, 2025

Sample information
Picture
Photos by: Ewa. A
Location
Collection date 09/04/2025
Captive / Cultivated? Wild-caught
Group Benedictine University
Observations

collected by Fall 2025 students.
Putative identification Arthropoda Insecta Hymenoptera Apidae Bombus Bombus terrestris

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit.
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes

Lab Manual

Results
Wolbachia presence No
Confidence level Low
Explanation of confidence level

my Wolbachia bands were not detectable, so no possibility of Wolbachia to exist in my insect.
Wolbachia 16S sequence Download FASTA   
>53-BW_G4_WoR (1).ab1 (327 bp) GGGTGATCCCGATTACTAGCGATTCCAGTTGCAGAGTACAATCCGAACTGAGATGTTTAAGATACTTACCTACTTTGCAG CCCATTGACACCATTGTAGCACGTGTGTAGCCCACTCCATAAGGGCCATGATGACTTGACATCATCCCCACCTTCCTCCA GTTTATCACTGGCAGTTTCCTTAAAGTCCCCAGCATTACCTGATGGAACTAAGGATGAGGGTTGCGCTCGTTGCGGGACT TAACCCAACATCTCACGACACGAGCTGACGACAGCCATGCAACCCTGTGGAAACCGGCGAACCGACCCTATCCCTTCGAA TAGGTAT
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence Download FASTA   
>81-BA_G4_CoR (2).ab1 (381 bp) AAACTGTAAAGGAAAGACTAATGATCAAACAAATAAAGATTCCCTGTCGATATTAATTATAAAATTCCCTAAATAAAAAC TCCACAGGGGAAAAAATCTAATCTACAATGCCTCAATCAAGGCTGTCCTACTCCTCTTTCTTCTAAGAATAAAGTTAAAT ATTTATCTGGTCCTGAGATTATCCCCAATTATAATAGGTATTACTATAAAAAAAATTATTACAAAAGCATGAGCAGTTAC AATTACATTGTAAATTTGATCATCTCCAATTAAGCTTCCAGGTTGTCCTAATTCAGTGCGAATAATTATCCTAAGAGCAG TCCCAACAGCCCCTGCTCAAACCTCCAAATACAAAATATAAGGTCCCAATATCTTTGATTA
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Bombus terrestris was found to be negative for Wolbachia.
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