Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 10/14/2025 |
| Captive / Cultivated? | Wild-caught |
| Group | Portland State University |
| Observations | |
| Putative identification | Arthropoda Insecta Coleoptera Carabidae Nebria Nebria brevicollis |
Methods |
|
| Extraction kit | DNeasy (Qiagen) blood and tissue kit |
| DNA extraction location | Partial abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | 1X TAE |
| DNA stain | GelRed |
| Gel images |
|
| Protocol notes | |
Results |
|
| Wolbachia presence | Yes |
| Confidence level | High |
| Explanation of confidence level | Gel B, wells 26-28. Well 26 is positive for insect DNA, well 27 is positive for Wolbachia positive control, and well 28 is positive for Wolbachia in the beetle. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Nebria brevicollis was found to be postive for Wolbachia. |
Portland State University – Nebria brevicollis
Portland State University – Culicidae
Portland State University – Formicidae
Portland State University – Staphylinidae
Portland State University – Polistes dominula