6F Devils coach horse Beetle

January 27, 2026

Sample information
Picture
Entry by: Landon H
Location
Collection date 09/02/2025
Captive / Cultivated? Wild-caught
Group St. Paul Academy and Summit School
Observations

I collected this specimen on a sunny day after school on September 2nd, around 4 pm, and it was around 60 degrees. Arthropod has 6 legs, Black, Long antennas, and 2 wings.
Putative identification Arthropoda Insecta Coleoptera Staphylinidae Ocypus Ocypus olens

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Abdomen
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system MiniPCR
Buffer TBE
DNA stain SeeGreen
Gel images
Protocol notes

followed Wolbachia project protocols

Results
Wolbachia presence No
Confidence level Medium
Explanation of confidence level
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Ocypus olens was found to be negative for Wolbachia.
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