6B

January 25, 2026

Sample information
Picture
Entry by: Lucy B
Location
Collection date 09/08/2026
Captive / Cultivated? Wild-caught
Group St. Paul Academy and Summit School
Observations

This Spider was collected in a garden from its web on a soil bed. In temperatures of around 70-76 degrees and it was mostly sunny.
Putative identification Arthropoda Arachnida Araneae

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Whole arthropod
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system MiniPCR
Buffer TBE
DNA stain SeeGreen
Gel images
Protocol notes

Followed Wolbachia Project Protocols

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level
Wolbachia 16S sequence
Arthropod COI sequence Download AB1
Summary The Araneae was found to be negative for Wolbachia.
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