Sample information
Picture	Entry by: Asian Lady Beetle
Location
Collection date	09/08/2025
Captive / Cultivated?	Wild-caught
Group	St. Paul Academy and Summit School
Observations	it was summer and I found it on a fern near some grass
Putative identification	Arthropoda Insecta Coleoptera Coccinellidae Harmonia Harmonia axyridis
Methods
Extraction kit	DNeasy (Qiagen) blood and tissue kit
DNA extraction location	Whole arthropod
Single or Duplex PCR	Single Reaction
Gel electrophoresis system	MiniPCR
Buffer	TBE
DNA stain	GelGreen
Gel images
Protocol notes
Results
Wolbachia presence	No
Confidence level
Explanation of confidence level
Wolbachia 16S sequence
Arthropod COI sequence
Summary	The Harmonia axyridis was found to be negative for Wolbachia.