House Cricket 8A

January 27, 2026

Sample information
Picture
Entry by: Adelaide D
Location
Collection date 09/08/2026
Captive / Cultivated? Captive / Cultivated
Group St. Paul Academy and Summit School
Observations

This cricket was bought live from Petco, so it is nutritionally enhanced, as it was originally meant to be fed to someone’s lizard. It was about 60-70(°F) where the crickets were stored.
Putative identification Arthropoda Insecta Orthoptera Gryllidae Acheta Acheta domesticus

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Abdomen
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system MiniPCR
Buffer TBE
DNA stain SeeGreen
Gel images
Protocol notes

Results
Wolbachia presence Unknown
Confidence level High
Explanation of confidence level

I am very confident that I don’t know if my arthropod has Wolbachia. Or, I’m not at all confident I could tell you whether my arthropod has Wolbachia.
Wolbachia 16S sequence
Arthropod COI sequence
Summary
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