Pill bug #1

Sample information

Picture
Entry by: Aidan R.
Location
Collection date 09/25/2025
Captive / Cultivated? Wild-caught
Group Rutgers Preparatory School
Observations

Found near a pond, under a rock around 8:30 in the morning.

Putative identification Arthropoda Malacostraca Isopoda Armadillidae

Methods

Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Edvotek Gel Electrophoresis
Buffer TAE
DNA stain Ethidium Bromide
Gel images
Protocol notes

Results

Wolbachia presence Yes
Confidence level High
Explanation of confidence level
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Armadillidae was found to be postive for Wolbachia.
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