Pillbug 2

January 12, 2026

Sample information
Picture
Entry by: NNC
Location
Collection date 09/25/2025
Captive / Cultivated? Wild-caught
Group Rutgers Preparatory School
Observations

It was wet & cloudy, under damp rock, near a path, temp 72 degrees F, 8:45 am, near Raritan River.
Putative identification Arthropoda Malacostraca Isopoda Armadillidae Armadillidium

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Edvotek Gel Electrophoresis
Buffer 1X TAE
DNA stain Ethidium Bromide
Gel images
Protocol notes

standard protocol

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

The lab we did gave results that made sense and we followed protocols exactly.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Armadillidium was found to be negative for Wolbachia.
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