Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 09/25/2025 |
| Captive / Cultivated? | Wild-caught |
| Group | Rutgers Preparatory School |
| Observations | It was wet & cloudy, under damp rock, near a path, temp 72 degree F, 8:45 am, near Raritan river. |
| Putative identification | Arthropoda Malacostraca Isopoda Armadillidae Armadillidium |
Methods |
|
| Extraction kit | DNeasy (Qiagen) blood and tissue kit |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Edvotek Gel Electrophoresis |
| Buffer | 1X TAE |
| DNA stain | Ethidium Bromide |
| Gel images |
|
| Protocol notes | Standard protocol |
Results |
|
| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | We successfully got arthropod DNA but the gel showed no wolbachia either time we ran it (2 times). This brought us to a clear result that the arthropod doesn’t have an infection. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Armadillidium was found to be negative for Wolbachia. |
Lucy B- Arthropod
Spider
Pavement Ant solomon
Soil Centipede