Hornet 1

January 14, 2026

Sample information
Picture
Entry by: NNC
Location
Collection date 09/29/2025
Captive / Cultivated? Wild-caught
Group Rutgers Preparatory School
Observations

Cloudy, 62 degrees F, 1:16 pm, slight wind. Found on the ground by gazebo. Caught on dirt and grass.
Putative identification Arthropoda Insecta Hymenoptera Vespidae

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Partial abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Edvotek Gel Electrophoresis
Buffer 1X TAE
DNA stain Ethidium Bromide
Gel images
Protocol notes

Standard protocol

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

We ran a gel twice, and it came up Wolbachia positive both times. This left us with a near certainty that it has Wolbachia.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Vespidae was found to be postive for Wolbachia.
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