Grasshopper 1

January 14, 2026

Sample information
Picture
Entry by: NNC
Location
Collection date 09/29/2025
Captive / Cultivated? Wild-caught
Group Rutgers Preparatory School
Observations

Cloudy, 62 degrees F, 1:35 pm, found in the grass outside, caught with tweezers.
Putative identification Arthropoda Insecta Orthoptera Gryllidae Velarifictorus

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Partial abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Edvotek Gel Electrophoresis
Buffer 1X TAE
DNA stain Ethidium Bromide
Gel images
Protocol notes

Standard protocol

Results
Wolbachia presence Unknown
Confidence level Low
Explanation of confidence level

We are not confident with this result because the gel yielded no arthropod DNA but a “yes” for Wolbachia DNA. We reran and didn’t find any arthropod DNA but still were receiving a false positive for Wolbachia.
Wolbachia 16S sequence
Arthropod COI sequence
Summary
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