Sample information
Picture	Entry by: Ethan R
Location
Collection date	09/17/2025
Captive / Cultivated?	Wild-caught
Group	Frontier High School
Observations
Putative identification	Arthropoda Insecta Diptera Limoniidae
Methods
Extraction kit	DNeasy (Qiagen)
DNA extraction location	Abdomen
Single or Duplex PCR	Single Reaction
Gel electrophoresis system	MiniOne
Buffer	1X TAE
DNA stain	GelGreen
Gel images
Protocol notes
Results
Wolbachia presence	Yes
Confidence level	Medium
Explanation of confidence level	Electrophoresis showed positive for wolbachia, sequence results were poor quality
Wolbachia 16S sequence	Download AB1
Arthropod COI sequence	Download AB1
Summary	The Limoniidae was found to be postive for Wolbachia.