Louise Arthropod (Boxelder Bug)

January 21, 2026

Sample information
Picture
Entry by: Louise M
Location
Collection date 08/07/2025
Captive / Cultivated? Wild-caught
Group St. Paul Academy and Summit School
Observations

The bug is black, with orange and yellowish patterns on its back. It was found in a dark and cooler place.
Putative identification Arthropoda Insecta Hemiptera Rhopalidae Boisea

Methods
Extraction kit pcr
DNA extraction location Abdomen
Single or Duplex PCR
Gel electrophoresis system
Buffer
DNA stain
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

Our PCR test was run accordingly and I had enough DNA collected
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Boisea was found to be negative for Wolbachia.
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