7/H

January 21, 2026

Sample information
Picture
Entry by: Louise M
Location
Collection date 08/07/2025
Captive / Cultivated? Wild-caught
Group St. Paul Academy and Summit School
Observations

The bug is black, with orange and yellowish patterns on its back. It was found in a dark and cooler place.
Putative identification Arthropoda Insecta Hemiptera Rhopalidae Boisea Boisea trivittata

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Whole arthropod
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system MiniPCR
Buffer TBE
DNA stain SeaGreen
Gel images
Protocol notes

Followed Wolbachia Project Protocols

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

Our PCR test was run accordingly and I had enough DNA collected
Wolbachia 16S sequence
Arthropod COI sequence Download AB1
Summary The Boisea trivittata was found to be negative for Wolbachia.
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