Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 11/05/2025 |
| Captive / Cultivated? | Wild-caught |
| Group | Hamburg Area High School |
| Observations | dome shaped beetle black spots hard shell covering wings white spots near eyes orange color |
| Putative identification | Arthropoda Insecta Coleoptera Coccinellidae |
Methods |
|
| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Duplex Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | 1X TAE |
| DNA stain | SYBR Safe |
| Gel images |
|
| Protocol notes | We extracted the samples DNA by crushing it in a test tube and centrifuging the sample. We then put the samples DNA into a gel electrophoresis chamber and sent the DNA to be analyzed. |
Results |
|
| Wolbachia presence | Yes |
| Confidence level | High |
| Explanation of confidence level | I am confident in my results because all of the controls worked as we suspected they would show up. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Coccinellidae was found to be postive for Wolbachia. |
Wolbachia Project
Alexander Devlin-Myrmica
Brookelynn Foote- Coccinellidae
Maile Bentz- Harmonia axyridis
Madison Adams – Harmonia axyridis