Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 03/02/2026 |
| Captive / Cultivated? | Wild-caught |
| Group | Berkshire Community College |
| Observations | 6 long skinny legs, body around 5 times smaller than legs. Color: Brown almost translucent. Appears to not be fully grown. |
| Putative identification | Arthropoda Arachnida Araneae Pholcidae Pholcus Pholcus phalangioides |
Methods |
|
| Extraction kit | Monarch DNA extraction (NEB) |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Duplex Reaction |
| Gel electrophoresis system | Edvotek Gel Electrophoresis |
| Buffer | 1X TAE |
| DNA stain | Cyber Stain |
| Gel images |
|
| Protocol notes | |
Results |
|
| Wolbachia presence | No |
| Confidence level | Medium |
| Explanation of confidence level | The DNA extraction could have been better, the bars were vague and hard to make out due to a small amount of DNA being extracted. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Pholcus phalangioides was found to be negative for Wolbachia. |
Centipede – MJAR
Fruitfly – MJAR
Ant – MJAR
Mosquito – MJAR
Bumblebee – MJAR