Cicada 41

Sample information

Picture
Photos by: Dr. Replogle
Location
Collection date 05/22/2021
Captive / Cultivated? Wild-caught
Group The Summit Country Day School
Observations
Putative identification Hexapoda Insecta Hemiptera Cicadidae Magicicada Magicicada septendecim

Methods

Extraction kit Thermoscientific GeneJet Genomic Purification
DNA extraction location Partial abdomen
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system MiniPCR
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

Results

Wolbachia presence No
Confidence level High
Explanation of confidence level
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Magicicada septendecim was found to be negative for Wolbachia.
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