Sample information |
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| Picture |
|
|---|---|
| Location | |
| Collection date | 12/11/2025 |
| Captive / Cultivated? | Wild-caught |
| Group | Coalinga High School |
| Observations | i found it dead in the sun on my front porch. it was about 6 degrees out. |
| Putative identification | Arthropoda Insecta Diptera Muscidae Musca Musca domestica |
Methods |
|
| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | MiniOne |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | follwed miniOne protocol. Our lab went pretty well for the most part. The only part that had went wrong is when we got our results for wolbachia our lines where all smudged. |
Results |
|
| Wolbachia presence | Unknown |
| Confidence level | Medium |
| Explanation of confidence level | medium confidence because our arthropod results worked but our wolbachia results didnt go as well. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | |
Parson Spider
Wasp
Caterpillar
Fruit Fly
Fly