DEM-SBM Coleoptera

April 13, 2026

Sample information
Picture
Entry by: Samuel M
Location
Collection date 02/09/2026
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations
  • Date: 02/09/26
  • Time: 1:15 P.M. 
  • Method: Sweep net
  • Habitat: grassy field
  • Location: outside Victor Hall
Putative identification Arthropoda Insecta Coleoptera

Methods
Extraction kit
DNA extraction location my hands and a 1.5ml tube
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

A DNA extraction kit of in-house reagents was used.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

The Gel electrophoresis showed that no Wolbachia was. present in my specimen, and there was no 16s rRNA gene in my sequence.
Wolbachia 16S sequence
Arthropod COI sequence
GTAGGAACATCGTTAAGTATTTTAATTCGGTTAGAATTAGGAACTAgatg AAGATTAATTGGAAACGACCAAATTTATAATATAATTGTTACAGCTCATG CTTTCATTATAATTTTCTTTATAGTAATACCTATTATAATTGGGGGTTTT GGAAATTGATTAGTTCCTTTAATAATTGGAGCTCCTGATATAGCATTTCC ACGATTAAATAACATAAGATTTTGACTTTTACCCCCTGCTTTAACTCTTT TAATTTTAAGAACAATCGTAGAAATAGGGGCAGGAACAGGATGAACTGTT TACCCTCCTCTTTCTTCTAATTTAACACATAATGGGCCTTCAGTAGATTT AGTGATTTTTAGTTTACATTTAGCAGGAATTTCCTCAATTTTAGGTGCAG TAAATTTCATTTCAACTATTATAAATATACGTCCATTTGGTATAATACTT GATAAAACTCCTTTATTTGTATGATCTGTTCTTATTACAGCAATTCTTTT ATTACTATCACTACCAGTTCTTGCAGGAGCAATTACTATACTATTAACTG ACCGAAACTTAAATTCTTCTTTTTTTGACCCAACCGGTGGGGGAGACCCA ATTTTATACCAACATTTATTTTGATTTTTTGG
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Coleoptera was found to be negative for Wolbachia.
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