DEM-LKA Hemiptera

April 13, 2026

Sample information
Picture
Entry by: Laila A
Location
Collection date 02/09/2026
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

Open field, dry grass, high grass, exposed dirt patches, light wind, sunny weather
Putative identification Arthropoda Insecta Hemiptera

Methods
Extraction kit
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used

Results
Wolbachia presence No
Confidence level Medium
Explanation of confidence level

Negative control was problematic, DNA sequencing was negative for Wolbachia
Wolbachia 16S sequence
Arthropod COI sequence
TGATCaggAATAGTAGGAATAATACTAAGTATAATTATCCGAATTGAATT GGCTCacccatgatcATTTTTAAAAAATGATCAAATATATAATGTAATTG TTACATCTCATGCTTTTATTATAATTTTCTTTATAGTTATACCAATCATA ATTGGAGGATTTGGAAACTGACTTCTACCTCTTATAATTGGAGCACCAGA TATAGCTTTTCCCCGGTTAAATAATATAAGATTTTGACTTTTACCTCCTT CATTAACAATATTATTATCAAGATCATTAATCGAAACAGGAGCAGGAACA GGATGAACAGTATATCCACCTTTATCTAGAATTACTCACCACTCAGGACC AAGAGTTGACATATCTATTTTCTCTTTACATTTAGCAGGAATTTCATCAA TCTTAGGAGCAGTTAATTTTATTACAACCGTAATTAACATACGACCTTCA GGAATAACATTTGAAAAGACACCTTTATTTGTTTGATCTATTTTAATCAC TGCAGTACTTTTACTAATTTCGTTACCTGTACTAGCAGGTGCAATTACTA TATTATTAACAGACCGAAATATTAATACATCATTTTTTGACCCAGCAGGT GGGGGAGACCCAATTCTTTACCAACATTTATTTTGATTTTTTGG
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Hemiptera was found to be negative for Wolbachia.
Report Inappropriate Post