DEM-FSM ( Hymenoptera)

April 13, 2026

Sample information
Picture
Entry by: Faith M
Location
Collection date 02/09/2026
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

 

Date of collection 2/9/2026
Time of Collection 1:40 pm
Location of collection (description) Outside of the Science Center

(trees, flowers, branches)
Description of environment(habitat) Terrestrial : grassland
Method of collection Dipnet
Putative identification Arthropoda Insecta Hymenoptera

Methods
Extraction kit
DNA extraction location Rear half
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

I have a high confidence in my negative Wolbachia result. The PCR did not produce a visible band for the Wolbachia gene, which suggests that Wolbachia DNA was not present in my insect sample. Additionally, the control reactions worked as expected, indicating that the PCR process itself was functioning properly.
Wolbachia 16S sequence
Arthropod COI sequence
GATCTGGATACTAGGATCATCAATGAGACTTATTATTCGAATAGAATTAA GATCCCcatgatcATGAATTAGCAATGATCAAATTTATAATACAATTGTT ACTAGTCATGCATTCCTAATAATTTTTTTTATAGTTATACCATTTTTAAT TGGAGGATTTGGAAATTGGCTTATTCCTTTAATACTAGGATCACCTGATA TAGCATTCCCCCGAATAAATAATATTAGATTTTGATTACTTCCTCCCTCA TTATTTATACTTTTATTAAGAAATTTATTTTATCCAAGACCAGGAACTGG ATGAACAGTATATCCACCATTATCAGCATATTTATATCATTCTTCACCTT CAGTAGATTTTGCAATTTTTTCTCTTCATATATCAGGAATTTCCTCAATT ATAGGATCATTAAACTTAATAGTTACAATTATAATAATAAAAAATTTTTC TATAAATTATGACCAAATTTCATTATTTCCATGATCAGTTTTTATTACAG CAATTTTATTAATTATATCATTACCTGTATTAGCTGGAGCAATTACTATA CTATTATTTGATCGAAATTTTAATACATCATTTTTCGATCCTATAGGAGG TGGAGATCCAATTCTTTATCAACATTTATTTTGATTTTTTGG
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Hymenoptera was found to be negative for Wolbachia.
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