April 13, 2026

Sample information
Picture
Entry by: Brogan O.
Location
Collection date 01/26/2026
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations
Putative identification Arthropoda Insecta Coleoptera

Methods
Extraction kit
DNA extraction location last third of the body
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used

Results
Wolbachia presence No
Confidence level Medium
Explanation of confidence level

Data was a little higher on the CO1 gene. If it was closer to the control, I would have more confidence but it makes me feel like something went wrong
Wolbachia 16S sequence
Arthropod COI sequence
TTtggATCATGAGCTGCAATACTTGGaACTTCATTAAGACTATTAATTCG GATAGAATTAGGAACCCCtTtttggttAATTGGGAACGACCAAATCTATA ACTCTATCGTcactgctCATGCTTTTATCATAATTTTCTTTATAGTTATA CCTATTATAATTGGAGGATTCGGAAATTGATTAGTTCCTCTAATACTAGG AGCTCCAGATATAGCTTTCCCTCGTATAAATAATATAAGATTTTGATTAC TTCCTCCTTCTATTTCATTACTATTAGCTAGAAGATTTATTGAGTCAGGA GCGGGAACAGGATGAACAGTTTACCCTCCTCTTTCCAGTAATATTGCCCA CAGTGGAGCCTCAGTAGATTTAACAATTTTTAGTCTTCATTTGGCAGGAA TCTCTTCAATTTTGGGGGCAGTTAATTTTATCTCTTCAATTATAAATATA CGAACCCCTGGAATAACAATAGAAAAAATACCTTTATTTGCTTGATCTGT AGGAATTACTGCCGTTCTATTACTTCTTTCTCTCCCAGTATTAGCAGGAG CAATTACAATACTTTTAACCGATCGAAATTTAAATACCTCATTTTTTGAC CCAACCGGAGGAGGAGACCCAATTTTATATCAACACTTATTTTGATTTTT TGGTCACC
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Coleoptera was found to be negative for Wolbachia.
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