Insect Specimen Observation

April 14, 2026

Sample information
Picture
Entry by: James K
Location
Collection date 01/27/2026
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

Forest-like, insect found in dirt and leaves
Putative identification Arthropoda Insecta Blattodea

Methods
Extraction kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA-kit of in-house reagents was used

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

After seeing the gel results, I know that my specimen is Wolbachia positive.
Wolbachia 16S sequence
CATACCTATTCGAAGGGATAGGGTCGGTTCGGCCGGATTTCACACAGGTGTTGCATGGCTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGTAACCCTCATCCTTAGTTACCATCAGGTAATGCTGGGGACTTTAAGGAAACTGCTAGTGATAAACTGGAGGAAGGTGGGGATGATGTCAAGTCATCATGGCCCTTATGGAGTGGGCTACACACGTGCTACAATGGTGGCTACAATGGGCTGCAAAGTCGCGAGGCTAAGCTAATCCCTTAAAAGCCATCTCAGTTCGGATTGTACTCTGCAACTCGATTGCATGAAGTTGGAATCGCTAGTAATCGTGGATCAGCATGCCACGGTGAATAC
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence
TTTATTTTATCTTCGGAGCTTGATCTGGAATGGTAGGGACATCTTTAAGAATATTAATCCGAGCAGAACTAAATCAACCTGGTTCATTAATTGGAGATGATCAAATCTATAATGTTATTGTAACGGCTCACGCTTTTGTTATAATTTTCTTTATAGTTATGCCAATTTTAATTGGAGGATTTGGAAATTGATTAGTACCTTTAATATTAGGTGCTCCTGATATAGCATTTCCTCGTATAAATAATATAAGTTTTTGACTTTTACCTCCATCTTTATCTCTTCTATTAGCCAGTAGCCTTGTCGAAAGAGGAGCTGGGACTGGTTGAACCGTATACCCTCCCTTAGCTAGAGGAATTGCTCATGCTGGAGCTTCAGTAGATTTAGCTATTTTCTCATTACATCTTGCAGGTGTATCATCAATTTTAGGTGCCGTAAATTTTATTTCAACAATTATTAACATGAAACCAATTAATATAAGCCCTGAACGAATTCCTTTATTTGTTTGGTCAGTAGGTATTACTGCATTATTATTATTATTATCCTTACCAGTTCTTGCAGGTGCAATTACAATATTATTAACAGACCGAAATTTAAATACTTCATTTTTTGACCCTGCAGGGGGAGGTGATCCTATTTTATATCAACATTTATTT
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Blattodea was found to be postive for Wolbachia.
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