Hempitera DEM-TMJ

April 14, 2026

Sample information
Picture
Location
Collection date
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

Box elder bug
Putative identification Arthropoda Insecta Hemiptera

Methods
Extraction kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer 1X TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used

Results
Wolbachia presence No
Confidence level Low
Explanation of confidence level

Wasn’t sure because the research was 25% having wolbachia
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Hemiptera was found to be negative for Wolbachia.
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