Sample information |
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| Picture |
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|---|---|
| Location | |
| Collection date | 01/22/2026 |
| Captive / Cultivated? | Wild-caught |
| Group | Georgia Southern University |
| Observations | We used a sweep net and our bare hands for collection by rocks and trees. |
| Putative identification | Arthropoda Insecta Dermaptera |
Methods |
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| Extraction kit | |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | Other |
| Gel images |
|
| Protocol notes | DNA extraction kit of in house reagents was used. |
Results |
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| Wolbachia presence | Unknown |
| Confidence level | Medium |
| Explanation of confidence level | I had a faint line on my gel for the Wolbachia being discovered in my insect. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
GCTTGATCGGGgatgGTAGGAACTAATTTGAGAATTTTAATTCGAACAGA GTTAGGTCAGCCTGGATCTTTAATTGGAGATGATCAGATTTATAATGTTA TTGTTACTGCTCACGCTTTTATTATAATTTTTTTTATGGTAATGCCAATT ATAATTGGAGGGTTTGGTAATTGATTAGTTCCTTTAATATTGAGAGCTCC AGATATAGCTTTCCCACGAATGAATAATATAAGATTTTGGTTATTACCTC CATCTCTGATTTTATTATTATCAGGGGGCCTAGTAGATAGAGGGGCTGGG ACAGGTTGGACAGTGTATCCTCCTTTATCATCAGTGATTTCTCATGGAGG GGCTTCAGTGGATATAACTATTTTTTCTTTACATTTGGCAGGGGTTTCTT CTATTTTGGGAGCCATTAATTTTATTACTACTGTGATTAATATACGTCCA GAAGGTTTAAAGCTTGATCGTGTTCCTTTATTTGTTTGATCAGTAGCTAT TACTGCTTTATTATTATTGCTTTCTTTACCGGTTTTAGCAGGAGCTATTA CGATATTATTAACTGATCGAAATTTAAATACATCTTTTTTTGATCCAGCC GGAGGCGGAGATCCTATTTTATATCAACATTTATTTTGATTTTTTGG
BLAST at The Wolbachia Project BLAST at NCBI
|
| Summary | |
DEM-AES – Hymenoptera
DEM-ORK Order Hymenoptera
(no title)
DEM-OKA Orthoptera
DEM-MGC Coleoptera