DEM-RNS Hymenoptera

April 15, 2026

Sample information
Picture
Location
Collection date 01/28/2026
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations
  • The insect was collected on the Georgia Southern Armstrong campus at around 2:00 PM.
  • The insect specimen was found on a walking trail nearby the ARC gym.
  • The environment was a diverse habitat with soil, moist logs, and lots of leaf-litter.
  • The dip-net (D-Net) was used along with a collection jar to scoop the insect specimen.
Putative identification Arthropoda Insecta Hymenoptera

Methods
Extraction kit
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

Excessive double checking and confirmations were used along with a multitude of evidence points supporting the negative presence of Wolbachia.
Wolbachia 16S sequence
Arthropod COI sequence
ATTGGTTCATCTATAAGAATAATCATCCGATTAGAACTAGGATCTTGtta tTCTCTAATTAATAATGATCAAATCTACAACTCCCTAGTTACTAGACACG CTTTTATTATAATTTTTTTTATAGTTATGCCCTTCATAATTGGGGGATTT GGAAATTTCTTAGTCCCCTTAATACTCGGCTCCCCTGATATGGCCTATCC TCGAATAAATAATATAAGATTTTGACTTCTACCACCTTCTCTAACTCTTT TACTTATCAGAAGATTTATTAATAGAGGAGTGGGGACAGGATGAACTATC TATCCACCACTAGCCTCCAATATTTTTCACAGAGGGGCCTCTATTGACCT ATCTATTTTTTCCTTACATATCGCCGGAATATCATCAATCTTAGGGGCTA TTAATTTTATTTCTACAATCATTAACATACACCATAAAAATTTTACTATA GATAAAATCCCCCTACTAGTCTGATCTATCCTTATTACAGCCATTCTTCT TTTACTTTCCCTCCCAGTTCTTGCAGGAGCTATTACAATATTACTAACTG ACCGTAATTTAAACACCTCCTTCTTCGATCCCTCGGGAGGGGGAGATCCC ATTCTATatcAACATTTATTCTGATTTTTTGGTCACC
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Hymenoptera was found to be negative for Wolbachia.
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