BORPARHemiptera

April 16, 2026

Sample information
Picture
Entry by: PhoebeR
Location
Collection date 01/27/2026
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

By the parking lot across from

the Science Center by a tree

• On a grass patch by a tree

under leaves and pine straw

• Dug/sifted through dirt
Putative identification Arthropoda Insecta Hemiptera

Methods
Extraction kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used.

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level
Wolbachia 16S sequence
ATAGTTGGGTCATCCTTAAGATGAATTATTCGAATTGAACTGGGCcaccc ttgtgcgtTTATTGGAGATGATCAAATTTATAATATAATTGTaacTgcTC ATGCATTTATTATAATTTTCTTTATAGTTATGCCAATTATAATTGGAGGT TTTGGTAATTGGCTAGTACCTTTAATAATTGGTGCACCAGATATAGCATT CCCCCGAATAAATAATATAAGTTTTTGGTTATTACCCCCATCATTAACCC TACTATTAAGAAGTAGCATGGTTGAAATAGGGGCAGGAACAGGTTGAACT GTTTATCCTCCATTATCAAGCAATATTTCACATAGAGGGGCTTCAGTAGA TTTAGCTATTTTTTCCCTCCATTTAGCAGGAGTTTCATCTATTTTAGGAG CAATTAATTTTATCTCAACTATTATAAATATACGCCCAGCAGGTATAATT CCTGAGCGAACTCCTCTCTTTGTATGATCAGTGGGAATTACTGCTCTTCT ATTACTTTTATCCTTACCTGTTTTAGCAGGGGCTATCACTATATTATTAA CTGATCGTAATTTTAATACTTCCTTTTTTGACCCCACTGGTGGAGGAGAC CCTATCCTTTATCAACACTTATTTTGATTTTTTGGTCACCctgaAGTTTA
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence
Summary The Hemiptera was found to be postive for Wolbachia.
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