Sample information
Picture	Entry by: Kayin D
Location
Collection date	03/05/2026
Captive / Cultivated?	Wild-caught
Group	Auburn University at Montgomery
Observations
Putative identification	Arthropoda Insecta Coleoptera Curculionidae Cnestus Cnestus mutilatus
Methods
Extraction kit	DNeasy (Qiagen) blood and tissue kit
DNA extraction location	Whole arthropod
Single or Duplex PCR	Duplex Reaction
Gel electrophoresis system	MiniOne
Buffer	1X TBE
DNA stain	GelGreen
Gel images
Protocol notes
Results
Wolbachia presence	No
Confidence level	High
Explanation of confidence level
Wolbachia 16S sequence
Arthropod COI sequence	Download FASTA    Download AB1
Summary	The Cnestus mutilatus was found to be negative for Wolbachia.