Sample information |
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| Picture |
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|---|---|
| Location | |
| Collection date | 04/05/2026 |
| Captive / Cultivated? | Wild-caught |
| Group | Scientists in Residence |
| Observations | A small spider was caught in its web, which it had constructed on deck stairs. |
| Putative identification | Arthropoda Arachnida Araneae Tetragnathidae Leucauge Leucauge venusta |
Methods |
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| Extraction kit | tweezers |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | |
| Gel electrophoresis system | MiniOne |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
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| Protocol notes | |
Results |
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| Wolbachia presence | Yes |
| Confidence level | High |
| Explanation of confidence level | The DNA extraction was successful, as evidenced by amplification of the arthropod COI. Both (+) and (-) controls worked. The Wolbachia 16S band was present. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence | Download FASTA
Download AB1
TTGGTGCTTGGGCTTCATAGTAGGGACAGCTATAAGTGTGCTAATTCGGATTGAGTTAGGTCAATCGGGAAGATTTTTAGGCGATGATCAATTGTATAATGTGATTGTAACAGCTCATGCTTTTATTATAATTTTTTTTATAGTTATACCTATTTTAATTGGGGGATTTGGAAATTGATTGGTTCCTTTAATATTAGGGGCTCCTGATATGGCTTTTCCTCGGATAAATAATTTAAGTTTCTGACTATTACCTCCTTCACTCTTTTTATTATTTATTTCTTCAATGGTAGATATAGGAGTGGGGGCTGGGTGAACTGTATACCCTCCTTTAGCTTCTTTAGAAGGGCATTCTGGTAGTTCTATGGATTTTGCTATTTTTTCTCTTCATTTAGCTGGGGCTTCTTCTATTATAGGGGCTATTAATTTTATTTCTACTATTTTTAATATACGGGTAAATGGAATAAGAATAGAAAAAGTTCCTTTATTTGTTTGATCTGTGTTAATTACAGCTGTTTTATTATTATTATCGCTCCCGGTTTTAGCAGGGGCGATTACTATATTGTTAACAGATCGTAATTTTAATACTTCTTTTTTTGATCCTTCTGGAGGAGGGGATCCAGTTCTTTTTCAACATTTATTTTGATTTTTNG
BLAST at The Wolbachia Project BLAST at NCBI
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| Summary | The Leucauge venusta was found to be postive for Wolbachia. |
Leucauge venusta
Umbrella Wasp
Fannia Canicularis
Oulema Obsucra JM2
Collembola