Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 01/21/2026 |
| Captive / Cultivated? | Wild-caught |
| Group | Benedictine University |
| Observations | collected by Fall 2025 students |
| Putative identification | Arthropoda Malacostraca Isopoda Armadillidae Armadillidium Armadillidium vulgare |
Methods |
|
| Extraction kit | DNeasy (Qiagen) blood and tissue kit. |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | SYBR Safe |
| Gel images |
|
| Protocol notes | |
Results |
|
| Wolbachia presence | Unknown |
| Confidence level | Low |
| Explanation of confidence level | Although BLAST showed a partial match to Wolbachia, there was no clear band in the gel and the sequence quality was low. This suggests that the signal was weak or possibly due to noise or contamination, so Wolbachia could not be confidently confirmed |
| Wolbachia 16S sequence | Download FASTA
Download AB1
|
| Arthropod COI sequence | Download FASTA
Download AB1
|
| Summary | |
Centipede – MJAR
Fruitfly – MJAR
Ant – MJAR
Mosquito – MJAR
Bumblebee – MJAR