Sample information
Picture	Entry by: ZainabR
Location
Collection date	09/03/2024
Captive / Cultivated?	Wild-caught
Group	Benedictine University
Observations	collected by Fall 2024 students
Putative identification	Arthropoda Insecta Orthoptera
Methods
Extraction kit	Qiagen DNeasy Blood and Tissue Kit
DNA extraction location	Reproduction
Single or Duplex PCR	Single Reaction
Gel electrophoresis system	Standard electrophoresis system
Buffer	TAE
DNA stain	SYBR Safe
Gel images
Protocol notes
Results
Wolbachia presence	No
Confidence level	Medium
Explanation of confidence level	I had a DNA band present, but no Wolbachia present.
Wolbachia 16S sequence
Arthropod COI sequence	Download FASTA    Download AB1
Summary	The Orthoptera was found to be negative for Wolbachia.