Locust Seed Beetle

April 28, 2026

Sample information
Picture
Entry by: Allison C
Location
Collection date
Captive / Cultivated? Wild-caught
Group College of Eastern Idaho
Observations
Putative identification Arthropoda Insecta Coleoptera Chrysomelidae Amblycerus Amblycerus robiniae

Methods
Extraction kit Edwards Buffer
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer 1X TAE
DNA stain SeeGreen
Gel images
Protocol notes

Standard CO1 primers did not amplify DNA, used Pco1F and LepR1 primers.

Results
Wolbachia presence Unknown
Confidence level Low
Explanation of confidence level
Wolbachia 16S sequence
Arthropod COI sequence
TAGCCGGAACCTCCTTAAGAATTTTAATTCGAGCTGAACTAGGAAACCCTGGTTCATTAATTGGAAACGACCAAATTTATAATGTAATCGTTACTGCTCATGCTTTTGTAATAATTTTTTTTATAGTAATACCTATTATAATTGGAGGATTCGGAAATTGACTTGTCCCTCTTATACTAGGAGCCCCAGACATGGCTTTCCCCCGAATAAATAATATAAGATTTTGACTTTTACCCCCTTCATTGACCCTTCTTTTAATAAGTAGAATTGTTGAAAATGGGGCAGGAACCGGTTGAACGGTTTATCCCCCTCTTGCCGCTAATATTGCTCACAGTGGATCTTCAGTTGATTTGGCTATTTTTAGTCTACACTTAGCGGGAATTTCTTCAATCCTAGGAGCTGTTAATTTTATTTCAACAGTTATTAATATACGACCAAAAGGAATAAGTCTTGATCGATTGCCATTATTTTCCTGAGCTGTAGTTATTACAGCAATTCTTTTACTTTTATCTCTACCTGTCTTAGCTGGAGCTATTACCATACTTTTAACTGATCGAAATTTAAATACAACTTTCTTTGATCCTGCTGGAGGAGGTGATCCTATTCTCTACCAACATTTATTCTGATTTTTT
BLAST at The Wolbachia Project   BLAST at NCBI
Summary
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