Aphid

April 30, 2026

Sample information
Picture
Entry by: Angie E.
Location
Collection date 09/01/2025
Captive / Cultivated? Wild-caught
Group Benedictine University
Observations

Freezer, SEP. 2025 on campus
Putative identification Arthropoda Insecta Hemiptera Aphididae

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes
  • Arthropod DNAP (708 bp) Yes Present
  • Wolbachia DNA (438 bp) Not Present

Results
Wolbachia presence Yes
Confidence level Medium
Explanation of confidence level

The gel electrophoresis showed negative, but the phylogenetic tree shows positive.
Wolbachia 16S sequence Download FASTA   
Arthropod COI sequence Download FASTA   
Summary The Aphididae was found to be postive for Wolbachia.
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