Formica incerta

April 29, 2026

Sample information
Picture
Entry by: Mustafa M
Location
Collection date 09/02/2025
Captive / Cultivated? Wild-caught
Group Benedictine University
Observations

Collected in fall 2025
Putative identification Arthropoda Insecta Hymenoptera Formicidae Formica Formica incerta

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

My confidence in the presence of wolbachia is high, however the gel electrophoresis results showed that wolbachia was not present, but the experiments run by to identify if we had DNA and wolbachia indicated that it was present. Because these results conflict, the gel likely was affected by experimental errors.
Wolbachia 16S sequence Download FASTA    Download AB1
TCATCCTTAGTTACCATCAGGTAATGCTGGGGACTTTAAGGAAACTGCCAGTGATAAACTGGAGGAAGGTGGGGATGATG TCAAGTCATCATGGCCCTTATGGAGTGGGCTACACACGTGCTACAATGGTGGCTACAATGGGCTGCAAAGTCGCGAGGCT AAGCTAATCCCTTAAAAGCCATCTCAGTTCGGATTGTACTCTGCAACTCGAGTGCATGAAGTTGGAATCGCTAGTAATCG TGGATCAGCACGCCACGGTGAATACGTTCTCGGGTCTTGTACACACTGCCCGTCACGCCATGGGAATTGGTTT
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence Download FASTA    Download AB1
AGTATAATTATTCGATTAGAATTAGGTTCTTCAAATTCATTAATTAATAATGATCAAATTTATAATTCTTTAGTAACTAA TCATGCCTTTATTATAATCTTTTTTATAGTAATACCATTTATAATTGGTGGATTTGGAAATTTTCTAATTCCTTTAATAT TAGGGTCACCAGATATAGCTTATCCTCGTATAAATAATATAAGATTTTGACTTTTACCTCCTTCCATCACTCTATTACTT TTAAGAAACTTTATTAATGATGGTACTGGAACTGGATGAACTATCTACCCTCCTTTAGCTTCAAATATTTTTCATAATGG ACCTTCTGTAGACTTAACAATTTTTTCTCTTCATATTGCCGGTATATCATCTATTTTAGGGGCTATTAATTTTATTTCAA CAATTATTAATATACATCATAAAAATTTTTCTATTGATAAAATTCCCCTACTTGTATGATCTATCTTAATCACTGCAATT TTACTTTTATTATCCCTTCCCGTTTTAGCTGGGGCTATTACAATATTATTAACTGACCGAAACTTAAATACTTCATTTTT TGACCCTTCAGGAGGGGGGGACCCAATTCTATATCAACATTTATTTTGATTTTT
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Formica incerta was found to be postive for Wolbachia.
Report Inappropriate Post