My info

May 3, 2026

Sample information
Picture
Entry by: Ayaan A
Location
Collection date 09/03/2025
Captive / Cultivated? Wild-caught
Group Benedictine University
Observations

Collected by Fall 2025 students
Putative identification Arthropoda Insecta Diptera Muscidae Musca Musca domestica

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

I have high confidence because there was a band in my gel results
Wolbachia 16S sequence Download FASTA    Download AB1
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAGCGGTAGAGAGGTAGCTTGCTACCTTGTTACGACTTCACCCCAGTCGTTTAGAGGGTGATGATGTAGCCGGCCTGAGAGGGTGACCGGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGACGGAGCAACGCCGCGTGAGTGATGAAGGTTTTCGGATCGTAAAACTCTGTTGTTAGAGAAGAACAAGGACGGTATCTTGACGGTACCTAACCAGAAAGCCACGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGTAGGCGGTTTGTTAAGTCAGATGTGAAATCCCCGGGCTCAACCTGGGAATTGCATTTGAAACTGGCAAGCTAGAGTACGGTAGAGGGTGGTGGAATTTCCTGTGTAGCGGTGAAATGCGTAGATATAGGAAGGAACACCAGTGGCGAAGGCGACTCTCTGGTCTGTAACTGACGCTGAGGCGCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGTCGACTAGCCGTTGGGAGTCTGGAGACGGTGGAACTTGCGAGAGTAGGTAGCTGGAGGAAGGCGGGGACGACGTCAAGTCATCATGGCCCTTACGGGCTGGGCTACACACGTGCTACAATGGCGGTACAACGAGTCGCAAGCCGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGAC
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence Download FASTA    Download AB1
AACTTTATATTTCATTTTTGGAGCTTGAGCAGGTATAGTAGGTACATCTTTAAGAATTTTAATTCGAGCAGAATTAGGTCATCCTGGAGCTTTAATTGGAAATGATCAAATTTATAACGTAATTGTTACAGCTCATGCTTTTATTATAATTTTTTTTATAGTAATACCTATTATAATTGGAGGATTTGGAAATTGATTAGTTCCTTTAATATTAGGAGCTCCAGACATAGCATTCCCCCGAATAAATAATATAAGTTTTTGACTTTTACCTCCAGCATTAACTTTATTATTAGTAAGTAGTATAGTAGAAAACGGAGCTGGGACAGGATGAACTGTTTACCCACCTTTATCATCAAATATTGCTCATGGTGGAGCTTCTGTAGATTTAGCTATTTTTTCTTTACACTTAGCAGGAATTTCTTCTATTTTAGGAGCTGTAAATTTTATTACAACTGTAATTAATATACGTTCTACAGGAATTACTTTTGACCGAATACCTTTATTTGTCTGATCAGTAGTAATTACAGCTTTATTATTATTGTTATCTTTACCAGTATTAGCAGGTGCTATTACTATATTATTAACAGACCGAAATTTAAATACTTCATTTTTTGACCCTGCAGGAGGAGGTGACCCAATTTTATATCAACATTTATTT
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Musca domestica was found to be postive for Wolbachia.
Report Inappropriate Post