Lucilia sericata

April 30, 2026

Sample information
Picture
Entry by: Shujaath A
Location
Collection date 09/02/2025
Captive / Cultivated? Wild-caught
Group Benedictine University
Observations

Collected by Fall 2025 students
Putative identification Arthropoda Insecta Diptera Calliphoridae Lucilia Lucilia sericata

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes

Results
Wolbachia presence Yes
Confidence level Low
Explanation of confidence level

The confidence level is low due to the poor quality of DNA which can be observed in the gel electrophoresis and the chromatogram.
Wolbachia 16S sequence Download FASTA    Download AB1
GTCAAGCCTTCTTGGCCCTAATGGAGGGGGTAACACACGGGTAACAAGGGGGGTTACAATGGGTTGAAAAGCGGGGGAGG CTAAACTAATCCCTTAAAACATCTCTCAGTTCGGATTGTACTCTGAAACTCGAGCG
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence Download FASTA    Download AB1
GAAATTGATTAGTTCCATTAATACTAGGAGCTCCAGATATAGCATTCCCTCGAATAAATAATATAAGTTTTTGACTTTTA CCTCCTGCATTAACTTTATTATTAGTTAGTAGTATAGTAGAAAACGGAGCTGGAACAGGATGAACAGTTTACCCTCCTCT ATCTTCTAATATTGCTCATGGAGGAGCTTCTGTTGATTTAGCTATTTTCTCTCTTCATTTAGCAGGAATTTCTTCAATTT TAGGAGCTGTAAATTTTATTACTACAGTTATTAATATACGATCAACAGGAATTACTTTTGATCGAATACCTTTATTTGTT TGATCAGTAGTAATTACAGCTTTATTACTTTTATTATCATTACCAGTATTAGCAGGAGCTATTACAATACTTTTAACAGA CCGAAATCTTAATACATCATTCTTTGA
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Lucilia sericata was found to be postive for Wolbachia.
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