Bee – 2 BASD

May 5, 2026

Sample information
Picture
Entry by: Kaylee S
Location
Collection date 08/25/2025
Captive / Cultivated? Wild-caught
Group Beaver Area School District
Observations

The temperature was about 64 degrees out and the bee was collected in a forest area. It was collected around 8 AM on August 25, 2025.
Putative identification Arthropoda Insecta Hymenoptera

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system BioRad
Buffer TAE
DNA stain UView
Gel images
Protocol notes

DNA Extraction: the abdomen of the bee was extracted for DNA.

Lane 1: Ladder

Lane 2: insect sample #1

Lane 3: insect sample #2

Lane 4: + fruit fly

Lane 5: – Fruit Fly

Lane 6: + DNA

Lane 7: – water

Analysis: all of the control bands worked. Some of lane six spilled over to lane seven so we moved lane 7’s water to lane 8.

Results
Wolbachia presence No
Confidence level Medium
Explanation of confidence level

Even though we did get results on lane four, lane six did not get a band in lane six. The insect samples did not test positive for Wolbachia.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Hymenoptera was found to be negative for Wolbachia.
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