Light Brown Apple Moth

May 6, 2026

Sample information
Picture
Entry by: Mahmood S
Location
Collection date 09/02/2025
Captive / Cultivated? Wild-caught
Group Benedictine University
Observations

Collected by Fall 2025 students from a container in the lab.
Putative identification Arthropoda Insecta Lepidoptera Tortricidae Epiphyas Epiphyas postvittana

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes

Arthropod PCR did not produce a band. Wolbachia band was faint but visible at ~430 bp. Standard lab protocol followed for all other steps.

Results
Wolbachia presence Yes
Confidence level Low
Explanation of confidence level

Choosing Low because the Wolbachia gel band was faint rather than strong. Although the BLAST match returned 100% identity to Wolbachia endosymbiont of Armadillidium vulgare (E-value 3e-95), the weak band intensity suggests low template DNA, which lowers confidence in whether the sequence reliably represents Wolbachia infection in this specific moth specimen. The low 260/230 ratio (1.39) from the nanodrop also indicated contamination that may have affected PCR efficiency.
Wolbachia 16S sequence Download FASTA    Download AB1
CTACAATGGGCTGCAAAGTCGCGAGGCTAAGCTAATCCCTTAAAAGCCATCTCAGTTCGGATTGCACTCTGCAACTCGAG
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence Download FASTA    Download AB1
CCGTTCGGTCAGTGATGATGTGTTAGAGTACAATGAAGTTTAGACTCTTATATCCACTTCATTAGCCGCCAAAATATCGA TGTTAAGAACGAACGATCTAATTTATAGTACCATTGTCACGGCGCTTGTTTTTATTATTTTTTTTTTACTAATCCTACCA ATTGTGGTGGGTTGATTAATTATTTGCTTCCTACCATTAAAAGCACGCGCTCCTTATCTATCTCTCCTCAAAAAAATGTG GAATGACCCTTCCCCCATCCCCCTCCCCTTCTATTTTAAATACACTAAGAAAAGGAGGGAACGGAAACTGGACGGGACTC CCCTCCTTCCCCTACTCTCTATCATAAATTGCACGGGGCGGCTGATATGTATTTTCTTTACTTTTCTCTATTGCCGAACA TGGAATCTTCAGATGTTATAATTTTATTTTTACAACCCTATCATCCCTATTTTCTTTTGATACATTGATCTTTGTCCTCA ATCGTATATCTATGATATTCACGATTATTACCTATCTTTATTCCCTTCCGGTTTTATCGTATTATATGGTAATATTACAA TATTATCAACTGTTTGTTCTCTCTCTATCTCCCTTCTGAAATTTAGGAGGAGTTTTTTTCTTTTACATTCTTATTTACTT TGCTTTTTGG
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Epiphyas postvittana was found to be postive for Wolbachia.
Report Inappropriate Post