Winterset Arthropod 3

May 26, 2026

Sample information
Picture
Entry by: Madelyn M
Location
Collection date 04/21/2026
Captive / Cultivated? Wild-caught
Group Winterset High School
Observations

Small little spider. Has a little body with long, thin legs that extend out. A beige color
Putative identification Arthropoda Arachnida Opiliones Sclerosomatidae Leiobunum Leiobunum vittatum

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniPCR
Buffer 1X TBE
DNA stain Cyber Green
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

In the photograph of the gel there is absolutely no wolbachia band present.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Leiobunum vittatum was found to be negative for Wolbachia.
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