Sample information
Picture	Entry by: Paeton C
Location
Collection date	04/21/2026
Captive / Cultivated?	Wild-caught
Group	Winterset High School
Observations	Daddy long leg
Putative identification	Arthropoda Arachnida Opiliones Sclerosomatidae Leiobunum Leiobunum vittatum
Methods
Extraction kit	DNeasy (Qiagen)
DNA extraction location	Abdomen
Single or Duplex PCR	Single Reaction
Gel electrophoresis system	MiniPCR
Buffer	1X TBE
DNA stain	Cyber Green
Gel images
Protocol notes
Results
Wolbachia presence	No
Confidence level	High
Explanation of confidence level	I followed protocol, the band was missing
Wolbachia 16S sequence
Arthropod COI sequence
Summary	The Leiobunum vittatum was found to be negative for Wolbachia.