winterset arthropod 14

May 26, 2026

Sample information
Picture
Entry by: Zachary J
Location
Collection date 04/21/2026
Captive / Cultivated? Wild-caught
Group Winterset High School
Observations

fat millipede with lots of legs

grey with tan/yellowish spots
Putative identification Arthropoda Diplopoda Polydesmida Euryuridae Euryurus Euryurus evides

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniPCR
Buffer 1X TBE
DNA stain Cyber Green
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level Medium
Explanation of confidence level

the protocol has been followed by all steps and no band observed
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Euryurus evides was found to be negative for Wolbachia.
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